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采用油品水融合组分(WAF)-发光细菌法对WAF制备过程中的搅拌速度、搅拌时间、搅拌温度、静置时间等影响因素进行优化,确定最佳检测条件,并对19种海上船载油品的急性生物毒性进行毒性等级分级研究。研究结果表明,发光细菌法能够检测出不同油品的急性生物毒性,不但具有良好的区分性,而且具有操作便捷、试验成本低和试验周期短等优点;在油品水融合组分(WAF)制备过程中的搅拌时间和搅拌温度对检测结果影响较大;水融合组分(WAF)检测的最佳条件为:搅拌时间为24 h,温度为25℃,搅拌速度为900 r/min,静置时间为1 h;海上船载油品的急性生物毒性是不同的,总体来看,重质燃料油>润滑油>柴油>原油。
The WAF-luminescent bacteria method was used to optimize the conditions of stirring speed, stirring time, stirring temperature, standing time and other factors during the preparation of WAF. The optimal detection conditions were determined. Acute toxicity of carrier oil Toxicity grade grading studies. The results showed that the light-emitting bacteria method can detect the acute toxicity of different oils, not only has good distinction, but also has the advantages of convenient operation, low test cost and short test period. In WAF, The optimum conditions for the determination of water fusion components (WAF) were as follows: the stirring time was 24 h, the temperature was 25 ℃, the stirring speed was 900 r / min, the static Set the time of 1 h; marine biological acute toxicity of cargo is different, on the whole, the heavy fuel oil> lubricant> diesel> crude oil.