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目的探讨microRNA-432(miR-432)在T2DM患者外周血清中的表达及其与IS的关系。方法收集在我院就诊的T2DM前期患者(n=19)、T2DM患者(n=23)和健康志愿者(NC组,n=20)外周血液标本,qRT-PCR检测miR-432水平,分析其与糖脂代谢、炎症因子及与IR的相关性。结果外周血中miR-432在NC组、T2DM前期组和T2DM组中呈下降趋势(1.00 vs 0.66 vs 0.35,P<0.05)。Pearson相关分析显示,miR-432与TC、LDL-C、载脂蛋白B/载脂蛋白A-1(ApoB/ApoA-1)、FPG、HOMA-IR、高敏C反应蛋白(hsC-RP)呈负相关,与ApoA-1、FIns和HDL-C呈正相关(P<0.05)。多元线性回归分析分析显示,BMI(β=0.465,95%CI=0.005~0.019,P=0.001)和miR-432(β=0.254,95%CI=0.039~0.146,P=0.045)是IS下降的独立影响因素。结论 miR-432在T2DM前期和T2DM患者外周血中下降,可能通过对糖脂代谢的影响参与IS的调节。
Objective To investigate the expression of microRNA-432 (miR-432) in peripheral blood of patients with T2DM and its relationship with IS. Methods Peripheral blood samples from T2DM patients (n = 23) and healthy volunteers (NC group, n = 20) were collected before treatment in our hospital. The levels of miR-432 were detected by qRT-PCR. And glucose and lipid metabolism, inflammatory factors and the correlation with IR. Results The level of miR-432 in peripheral blood was decreased in NC group, pre-T2DM group and T2DM group (1.00 vs 0.66 vs 0.35, P <0.05). Pearson correlation analysis showed that miR-432 was positively correlated with TC, LDL-C, ApoB / ApoA-1, FPG, HOMA- IR and hsC-RP Negative correlation with ApoA-1, FIns and HDL-C was positively correlated (P <0.05). Multivariate linear regression analysis showed that BMI (β = 0.465, 95% CI = 0.005-0.019, P = 0.001) and miR-432 (β = 0.254, 95% CI = 0.039-0.1446, P = 0.045) Independent factors. Conclusion miR-432 is decreased in the peripheral blood of pre-T2DM and T2DM patients, and may be involved in the regulation of IS through the influence of glucose and lipid metabolism.