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为了深入探究木质素降解酶在不同堆肥基质中的吸附传输特性,通过批量实验对比了土壤、菜叶、稻草和米糠对木质素降解酶的吸附性能,并进行了动力学分析和等温吸附模型拟合,同时通过柱淋洗实验考察了木质素降解酶在4种堆肥基质中的迁移传输特性.结果表明,堆肥基质对木质素降解酶的吸附与基质种类有关,土壤、菜叶、稻草和米糠对木素过氧化物酶(LiP)和锰过氧化物酶(MnP)的吸附量分别为1.22、1.27、1.13、1.22U·g-1和5.09、4.88、4.43、3.95U·g-1.比较LiP和MnP吸附的动力学模型,准二级动力学方程为表征木质素降解酶吸附的最佳模型,其R2值为0.9732～0.9997,Elovich方程拟合较差,准一级动力学方程拟合最差;Langmuir模型对等温吸附数据进行拟合效果最好,而实验数据不适合用Freundlich方程表征.土壤、菜叶、稻草和米糠对LiP和MnP饱和吸附容量分别为1.23、1.30、1.17、1.14U·g-1和5.70、5.19、4.73、4.14U·g-1.LiP和MnP在稻草和米糠基质中传输效果较好,可传输到最深层10mL处,而在土壤和菜叶基质中则被滞留在浅层.
In order to further explore the adsorption and transport characteristics of ligninolytic enzymes in different compost mats, the adsorption properties of ligninolytic enzymes by soil, leaves, straw and rice bran were compared by batch experiments. The kinetic analysis and isothermal adsorption model The results showed that the adsorption of ligninolytic enzymes by the compost matrix was related to the types of substrates, and the soil, leaves, straw and rice bran The adsorption amounts of lignin peroxidase (LiP) and manganese peroxidase (MnP) were 1.22,1.27,1.13,1.22U · g-1 and 5.09,4.88,4.43,3.95U · g-1, respectively. Compared with the kinetic model of LiP and MnP adsorption, the quasi-second-order kinetic equation is the best model to characterize the adsorption of ligninolytic enzymes. The R2 value is 0.9732-0.9997. The Elovich equation is poorly fitted. The quasi-first-order kinetic equation And Langmuir model fitted the best adsorption isotherm data, while experimental data were not suitable for characterization by Freundlich equation.The saturated adsorption capacities of LiP and MnP in soil, leaves, rice straw and rice bran were 1.23,1.30,1.17, 1.14U · g-1 And 5.70,5.19,4.73,4.14U · g-1.LiP and MnP in the straw and rice bran matrix transport better, can be transmitted to the deepest 10mL at the soil and leaves of the matrix was stuck in the shallow layer .