【摘 要】
:
精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义. 以大斑病的感病自交系"获白"为母本, 抗病自交系"77
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精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义. 以大斑病的感病自交系"获白"为母本, 抗病自交系"77Ht2"为父本,构建了F2作图群体. 通过RFLP分析知, Ht2基因定位在第8染色体的UMC89和BNL2.369之间, 与BNL2.369相距0.9 cM. 然后选用在该区域的SSR标记进行了连锁分析, 确定了SSR标记UMC1202, BNLG1152, UMC1149与Ht2基因紧密连锁,
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