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亲水性量子点的荧光性能是其作为生物检测探针的一个重要质量指标.不同结构的量子点在亲水性修饰过程中,其抵抗荧光淬灭的能力差异较大.设计与制备具有不同结构和成分的核、核壳量子点,再通过双亲性高分子对其亲水性改性,利用荧光光谱监测亲水性修饰过程中的荧光性能变化来度量所合成量子点的光化学稳定性.实验结果表明,在表面亲水性修饰过程中,未包覆壳层的裸核量子点其抵抗荧光淬灭的能力最弱;包覆壳层的核壳量子点,其抵抗荧光淬灭的能力增强,且壳层越多,抵抗能力越强.壳层的结构和成分直接影响核壳量子点抵抗荧光淬灭的能力,具有合理晶格匹配的核壳量子点,其抵抗荧光淬灭的能力较强.另外,通过优化设计与制备的核壳量子点经表面亲水性修饰后,再偶联叶酸,构建出特异性生物荧光探针,对乳腺癌细胞进行靶向性标记后,利用流式细胞仪进行细胞检测分析.实验结果表明,通过优化制备的核壳量子点,亲水性修饰后仍具有很好的荧光性能,偶联叶酸后具有较好的细胞靶向性.
Fluorescence properties of hydrophilic quantum dots are an important quality indicator for biosensing probes. Quantum dots of different structures have different capacities to resist fluorescence quenching during hydrophilic modification. Structure and composition of core and shell quantum dots, and then hydrophilic modification by amphiphilic macromolecules, the use of fluorescence spectroscopy to monitor changes in the fluorescence of hydrophilic modification process to measure the photochemical stability of the synthesized quantum dots. The experimental results show that the uncoated shell bare nuclei QDs have the weakest ability to resist fluorescence quenching during surface hydrophilic modification. The core-shell QDs that coat the shell are resistant to fluorescence quenching And the more the shell, the stronger the resistance.The structure and composition of the shell directly affect the ability of the core-shell quantum dots to resist fluorescence quenching, the lattice-matched quantum dots with reasonable lattice matching, which resist the ability of fluorescence quenching Strong.Furthermore, by optimizing the design and preparation of core-shell quantum dots modified by surface hydrophilicity, coupled with folic acid, a specific biofluorescence probe was constructed to target the breast cancer cells, Cells Cells were detected and analyzed. The results show that, by optimizing the core-shell quantum dots prepared after hydrophilically modified still has a good fluorescent properties, preferably after coupling with folic acid targeting a cell.