1979年我们对水稻各部分器官(幼根、幼茎、叶片、幼穗及茎尖)离体培养和分化苗的再利用进行试验,取得了初步结果,已诱发出大量愈伤组织及绿苗。这一无性繁殖方法对加速优良品种和来源困难材料的繁殖,尤其对杂交水稻亲本提纯和扩大繁殖提供了一条有效途径。供试材料粳稻品种:银河、辰巳糯、黄金利、恢复系C_(290)、不育系74—134(再生稻苗)。诱导方法首先在无菌条件 In 1979, we tested the reutilization of in vitro and differentiated shoots of various organs of rice (young roots, young stems, leaves, young spikes and shoot tips) and obtained preliminary results. A large amount of callus and green seedlings have been induced . This clonal propagation method provides an effective way to accelerate the propagation of difficult and difficult-to-source materials, especially for the purification and propagation of hybrid rice parents. The japonica rice cultivars were as follows: Yinhe, Tatsunami, Huanglili, restorer C_ (290), CMS line 74-134 (regenerated rice seedlings). Induction methods are first performed under sterile conditions