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目的研究番茄红素(Lycopene,LP)对人肝癌HepG2细胞生长的影响并初探其机制。方法取对数生长期HepG2细胞设空白对照组、LP(5μg/mL)组、LP(10μg/mL)组、LP(20μg/mL)组和顺铂(40μg/mL)组,每组设10个复孔;各组分别给药干预48 h后,噻唑蓝(MTT)比色法测定细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和细胞凋亡状况,RT-PCR法检测Bax mRNA和Bcl-2 mRNA表达,Western blot法检测Caspase-3蛋白表达。结果与空白对照组比较,经LP(10μg/mL、20μg/mL)或顺铂40μg/mL干预48 h能够显著提高HepG2细胞增殖抑制率,延长细胞周期中G_0/G_1期而缩短G_2/M期,提高细胞凋亡率,上调促凋亡Bax mRNA表达并下调抑凋亡Bcl-2 mRNA表达,提高Bax/Bcl-2比值,上调Caspase-3蛋白表达,LP上述作用具有一定的剂量依赖性。结论 LP具有抑制人肝癌HepG2细胞增殖并促进其凋亡的作用,其机制可能与LP干预细胞周期分布和调节凋亡相关基因蛋白表达有关。
Objective To study the effect of lycopene (LP) on the growth of HepG2 human hepatoma cells and to explore its mechanism. Methods HepG2 cells were divided into blank control group, LP group (5μg / mL), LP group (10μg / mL), LP group (20μg / mL) and cisplatin group After 48 h of intervention, the cell proliferation inhibition rate was determined by MTT assay, the cell cycle and apoptosis were detected by flow cytometry (FCM), and the expression of Bax by RT-PCR The mRNA and protein expression of Bcl-2 were detected by Western blot. Results Compared with the blank control group, the intervention of LP (10μg / mL, 20μg / mL) or cisplatin (40μg / mL) for 48 h could significantly increase the inhibition rate of HepG2 cells, prolong the G_0 / G_1 phase and shorten the G_2 / M phase , Increase the rate of apoptosis, up-regulate the expression of pro-apoptotic Bax mRNA and down-regulate the expression of Bcl-2 mRNA, increase the Bax / Bcl-2 ratio and upregulate Caspase-3 protein expression. Conclusion LP can inhibit the proliferation and promote the apoptosis of HepG2 cells. The mechanism may be related to the effects of LP on the cell cycle distribution and the regulation of the expression of apoptosis related genes.