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【目的】探讨毛橘红总黄酮提取物对酒精性肝损伤的保护作用。【方法】采用二锅头灌胃4周,结合喂养高脂饲料复制慢性酒精性肝损伤大鼠模型,造模同时分别给予不同浓度毛橘红总黄酮提取物,分别检测各组动物血清谷丙转氨酶(AST)、谷草转氨酶(ALT)含量,采用酶联免疫吸附(ELISA)法检测各组大鼠血清中肿瘤坏死因子-α(TNF-α)、转化生长因子β1(TGF-β1)和白细胞介素6(IL-6)的含量。【结果】与空白对照组比较,模型组ALT及AST含量显著升高(P<0.01),与模型组比较,不同剂量毛橘红总黄酮(217 mg/kg、433 mg/kg、108 mg/kg)均可不同程度降低ALT、AST、TNF-α、TGF-β1和IL-6的含量。【结论】毛橘红总黄酮可降低炎性细胞因子TNF-α、TGF-β1和IL-6的含量,对慢性酒精性肝损伤具有一定的保护作用。
【Objective】 To investigate the protective effect of the extract of total flavonoids from Mao orange on alcoholic liver injury. 【Method】 The model of chronic alcoholic liver injury was duplicated with high-fat diet by using Erguotou gavage for 4 weeks. At the same time, the total flavonoids of Fructus Margarita were respectively given to the rats. The levels of alanine aminotransferase (AST) ) And aspartate aminotransferase (ALT) were determined. Serum levels of tumor necrosis factor-α (TNF-α), transforming growth factor β1 (TGF-β1) and interleukin 6 (IL-6) were detected by enzyme linked immunosorbent assay (ELISA) (IL-6) content. 【Results】 Compared with the blank control group, the levels of ALT and AST in the model group were significantly increased (P <0.01). Compared with the model group, the total flavonoids of Mao Honghong (217 mg / kg, 433 mg / kg, 108 mg / kg ) Can reduce the levels of ALT, AST, TNF-α, TGF-β1 and IL-6 to varying degrees. 【Conclusion】 Total Flavonoids from Fructus Mume could reduce the content of inflammatory cytokines TNF-α, TGF-β1 and IL-6, and have a protective effect on chronic alcoholic liver injury.