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煤层气是一种重要的能源资源,有生物成因和热成因两种类型。生物成因煤层气是在煤层微生物的作用下形成的。本研究通过向煤层气井中注入培养基,采用宏基因组学技术对煤层水中细菌菌群的组成多样性进行分析。结果表明,在培养基注入前后,煤层水中微生物均具有较丰富的多样性,注入前丛毛单胞菌科、甲基球菌科、假单胞菌科、鞘氨醇单胞菌科和嗜甲基菌科的微生物为优势菌种。注入3个月后,优势菌种主要为链球菌科、丛毛单胞菌科、嗜甲基菌科和红环菌科。注入6个月后,红环菌科和甲基球菌科的丰度最高。主成分分析显示,红环菌科、甲基球菌科、弯曲杆菌科、鞘氨醇单胞菌科、假单胞菌科、丛毛单胞菌科、嗜甲基菌科和链球菌科8个科的微生物对生物成气过程有重要影响。细菌菌群的ɑ多样性从chao1、ACE、Shannon、npS hannon和Simpson指数进行分析,结果显示,培养基的注入能显著增加物种的多样性。本研究结果为解析煤层生物成气过程中微生物的动态变化提供了依据。
Coal bed methane is an important energy resource, which has two types, biological genesis and thermal genesis. Biogenic CBM is formed by the action of coal seam microorganisms. In this study, through the injection of culture medium into coalbed methane wells, metagenomic techniques were used to analyze the compositional diversity of bacterial flora in coal seams. The results showed that before and after the medium was infused, the microorganisms in the coal seam water were rich in diversity. Before injection, the microorganisms of the genus Comamonas, Methylococcus, Pseudomonas, Sphingomonas, Microbial strains of the dominant bacteria are predominant species. After 3 months of injection, the predominant species were Streptococcus, Comamonastrum, Methylophilus and Rhodococcus. Rhodocystins and Methylobacteria have the highest abundance after 6 months of injection. Principal component analysis showed that the strains of Rhodocytheria, Methylococcus, Campylobacter, Sphingomonas, Pseudomonas, Comamonas, Methylophilus and Streptococcus 8 The microorganism of each family has important influence on the bio-gas process. The diversity of bacterial flora was analyzed from chao1, ACE, Shannon, npS hannon and Simpson index. The results showed that the medium injection significantly increased species diversity. The results of this study provide the basis for analyzing the dynamic changes of microorganisms in the process of biogenic gas in coal seam.