Three fructans (CoPS1, CoPS2 and CoPS3) were isolated fromthe root of Cyathula officinalis Kuan, a traditional Chinesemedicine. The structures of the fructans were determined bymethylation, reductive-cleavage method combined with GC-MSahalysis, and ~(13)C NMR spectroscopy. These results show thatthe fructans (CoPS1, CoPS2 and CoPS3) are graminan typefructans, and comprised of (2→1)-and (2→6)-linked β-D-fructofuranosyl backbone residues containing high branches. The structures of the fructans were determined by methylation, reductive-cleavage method combined with GC-MS ahalysis, and ~ (13) C NMR spectroscopy. These results show that the fructans (CoPS1, CoPS2 and CoPS3) are graminan type fructans, and comprised of (2 → 1) -and (2 → 6) -linked β-D-fructofuranosyl backbone residues containing high branches.