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目的探讨麻黄、五味子对肺纤维化大鼠血管新生影响差异的作用机制。方法选择SPF级Wistar雄性大鼠90只,随机设立假手术组、模型组、氢化可的松组、麻黄组、五味子组以及麻黄五味子组。采用博莱霉素A5(7 mg/kg)气管滴入方法建立肺纤维化模型,假手术组灌胃生理盐水(6 mL/kg)28 d;中药水煎液浓缩至每毫升含原药材1 g,麻黄组、五味子组分别灌胃麻黄(5 g/kg)、五味子(2.5 g/kg)水煎液28 d,麻黄五味子组灌胃麻黄水煎液(5 g/kg)7 d后灌胃五味子水煎液(2.5 g/kg)至28 d,氢化可地松组肌注氢化可地松20 mg/kg28 d。于7、28 d放射免疫法检测各组大鼠内皮素-1(ET-1)、6-酮-前列腺素F1a(6-Keto-PGF1a)含量变化,免疫组化法观察肺组织微血管密度。结果 7 d时,模型组血浆ET-1含量升高,较假手术组差异有统计学意义(P<0.05),6-Ke-to-PGF1a含量较假手术组有所升高,但差异无统计学意义;28 d时,麻黄五味子组与氢化可的松组血浆ET-1含量显著降低,与模型组比较差异有统计学意义(P<0.05),各治疗组6-Keto-PGF1a含量较7 d时均有所升高。模型组肺微血管密度显著多于假手术组及各治疗组(P<0.05),麻黄组对肺小动脉血管新生的抑制高于五味子组、麻黄五味子组。结论麻黄对改善肺纤维化大鼠肺系数有积极作用,麻黄、五味子联合应用对肺纤维化的肺部小动脉损伤有抑制作用。
Objective To explore the mechanism of action of ephedra and schisandrae on the difference of angiogenesis in pulmonary fibrosis rats. Methods Ninety SPF Wistar male rats were randomly divided into sham operation group, model group, hydrocortisone group, ephedra group, Schisandra group, and ephedra schisandrae group. Pulmonary fibrosis model was established by intratracheal instillation of bleomycin A5 (7 mg / kg). Rats in sham operation group were given saline (6 mL / kg) for 28 days. The decoction of Chinese medicine was concentrated to 1 g, ephedra group and schisandrae group were treated with ephedra (5 g / kg) and Schisandra chinensis (2.5 g / kg) decoction for 28 days, ephedra schisandra chinensis group was treated with ephedra Ephedra Decoction (5 g / kg) Schisandra decoction (2.5 g / kg) to 28 d, hydrocortisone group intramuscular hydrocortisone 20 mg / kg 28 d. The levels of ET-1 and 6-Keto-PGF1a in rats were detected by radioimmunoassay at 7 and 28 days. The microvessel density of lung tissue was detected by immunohistochemistry. Results At 7 days, the content of ET-1 in model group was significantly higher than that in sham operation group (P <0.05), while the content of 6-Ke-to-PGF1a was higher than that in sham operation group Statistical significance; At 28 d, the content of ET-1 in the ephedra Schisandra and hydrocortisone group was significantly lower than that in the model group (P <0.05), and the content of 6-Keto-PGF1a 7 d have increased. The microvessel density in the model group was significantly more than that in sham operation group and each treatment group (P <0.05). The inhibition of angiogenesis of pulmonary arteriole in ephedra group was higher than that in schisandra chinensis and schisandra chinensis group. Conclusion Ephedra play a positive role in improving lung coefficient of pulmonary fibrosis in rats. Combination of ephedra and schisandrae can inhibit the damage of pulmonary arterioles in pulmonary fibrosis.