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目的采用小鼠骨髓细胞微核试验,对脱氢乙酸进行体内致突变安全性评价。方法按照1/10 LD50、1/5 LD50和1/2 LD50设置三个剂量组,另设置阴性对照组和环磷酰胺(CP)阳性对照组(100 mg·kg-1)。每组10只小鼠,雌雄各半,灌胃给药,两次攻毒时间间隔24 h。在第二次灌胃后6 h,处死小鼠,制片,观察并计数嗜多染红细胞的微核率,并与阳性对照组和隐形对照组进行比较分析。结果 1/5 LD50、1/10 LD50剂量时,与正常对照组无显著差异;1/2 LD50剂量组与正常对照组有显著差异(P<0.05),但无剂量-效应关系。结论脱氢乙酸小鼠骨髓细胞微核试验结果为阴性。
Objective To evaluate the in vivo mutagenic safety of dehydroacetic acid using mouse bone marrow cell micronucleus test. Methods Three dose groups were set up according to 1/10 LD50, 1/5 LD50 and 1/2 LD50, and negative control group and cyclophosphamide (CP) positive control group (100 mg · kg -1) were set. Each group of 10 mice, male and female, gavage, twice a time interval of 24 h. Six hours after the second gavage, the mice were sacrificed and the mice were sacrificed. The micronucleus rate of polychromatic erythrocytes was observed and counted, and compared with the positive control group and the stealth control group. Results LD50 and 1/10 LD50 showed no significant difference compared with normal control group. There was a significant difference (P <0.05) between 1/2 LD50 dose group and normal control group, but no dose-effect relationship. Conclusion Dehydroacetic acid mouse bone marrow cells micronucleus test results were negative.