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目的研究白介素27(IL-27)对THP-1单核细胞系Ⅱ类转录活化子(classⅡtransactivator,CⅡTA)和Ⅱ类主要组织相容性复合物(major histocom patibility complex classⅡ,MHCⅡ)分子的表达的影响及Toll样受体(Toll-likereceptor,TLR)激动剂LPS和Pam3CSK4的干预作用。方法RT-PCR检测CⅡTAⅠ、Ⅲ和Ⅳ以及人白细胞抗原(human leukocyte antigen,HLA)-DRA和干扰素调节因子-1(interferon regulatory factor-1,IRF-1)mRNA的表达。半定量PCR检测HLA-DRA、DRB、DPA、DPB、DQA、DQB、IRF-1、CⅡTA mRNA的表达。流式细胞术检测细胞表面HLA-DR的表达。结果IL-27刺激24h后可分别上调THP-1细胞CⅡTAⅢ、CⅡTAⅣ和IRF-1mRNA表达水平。IL-27刺激24h和48h可升高MHCⅡ类分子mRNA的表达,并能诱导HLA-DR在28%和53%的THP-1细胞表面表达。在THP-1细胞和PMA诱导的THP-1巨噬细胞,LPS和Pam3CSK4均可抑制IL-27诱导的CⅡTA和HLA-DR的表达。结论IL-27可上调THP-1细胞CⅡTA和MHCⅡ类分子的表达,这种作用可被LPS和Pam3CSK4抑制。
Objective To investigate the effect of interleukin 27 (IL-27) on the expression of classⅡtransactivator (CⅡTA) and major histocompatibility complex class Ⅱ (MHCⅡ) molecules in THP-1 monocytes, Effects and Toll-like receptor (Toll-like receptor, TLR agonists LPS and Pam3CSK4 intervention. Methods RT-PCR was used to detect the expression of CⅡTAⅠ, Ⅲand Ⅳ and the mRNA of human leukocyte antigen (HLA) -DRA and interferon regulatory factor-1 (IRF-1). Semi-quantitative PCR was used to detect the expression of HLA-DRA, DRB, DPA, DPB, DQA, DQB, IRF-1 and CⅡTA mRNA. Flow cytometry was used to detect the expression of HLA-DR on the cell surface. Results IL-27 stimulated the expression of CⅡTAⅢ, CⅡTAⅣ and IRF-1 mRNA in THP-1 cells after 24 h. IL-27 stimulation at 24h and 48h increased the expression of MHC class II mRNA and induced the expression of HLA-DR on 28% and 53% of THP-1 cells. In both THP-1 cells and PMA-induced THP-1 macrophages, both LPS and Pam3CSK4 inhibited IL-27-induced CⅡTA and HLA-DR expression. Conclusion IL-27 can up-regulate the expression of CⅡTA and MHCⅡ in THP-1 cells, which can be inhibited by LPS and Pam3CSK4.