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为探讨苯系物对男工精子染色体数目畸变的影响,对男工妻子流产率增加提供可能的机理。用X,Y着丝点染色体特异DNA探针(分别用生物素和地高辛标记)同时与人间期精子核杂交,用CY3-链亲和素检测X探针的杂交信号(红色),用抗地高辛抗体-荧光素检测Y探针杂交信号
In order to explore the effect of benzene series on the number of male workers’ sperm chromosome aberration, it provided a possible mechanism for the increase of abortion rate of male workers and wives. The X, Y centromere-specific DNA probes (labeled with biotin and digoxigenin, respectively) were simultaneously hybridized to human interphase spermatozoa, the hybridization signal of the X probe was detected with CY3-streptavidin (red) Anti-digoxin antibody-fluorescein Y probe hybridization signal