目的:探讨子宫内膜癌患者癌组织中PCNA和CD34蛋白的表达及意义。方法:选取子宫内膜癌手术患者120例,术后取手术切除的癌组织(实验组)及癌旁正常组织(对照组),采用ELISA法和实时荧光定量PCR法检测患者癌组织中PCNA和CD34蛋白的表达。结果:与对照组比较,实验组患者癌组织中PCNA、CD34的蛋白含量及m RNA水平均明显升高,差异有统计学意义(P<0.01);PCNA和CD34在组织中蛋白含量及m RNA水平均呈正相关性(r=0.608、P=0.037,r=0.576、P=0.029)。结论:子宫内膜癌患者癌组织中PCNA和CD34蛋白表达的升高,可能与子宫内膜癌的发生、发展有关,其作用机制有待于进一步研究。 Objective: To investigate the expression and significance of PCNA and CD34 protein in cancer tissue of patients with endometrial cancer. Methods: Totally 120 patients with endometrial carcinoma were selected for surgical resection of cancer tissue (experimental group) and adjacent normal tissue (control group). The levels of PCNA and PCNA were detected by ELISA and real-time fluorescence quantitative PCR CD34 protein expression. Results: Compared with the control group, the protein and mRNA levels of PCNA and CD34 in the experimental group were significantly increased (P <0.01). The protein and mRNA levels of PCNA and CD34 The levels were positively correlated (r = 0.608, P = 0.037, r = 0.576, P = 0.029). Conclusion: The increased expression of PCNA and CD34 protein in endometrial carcinoma may be related to the occurrence and development of endometrial carcinoma. The mechanism of action needs to be further studied.