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目的:建立舒经跌打丸的质量标准。方法:采用薄层色谱法对舒经跌打丸中三七药材进行定性鉴别,采用高效液相色谱法测定舒经跌打丸中大黄酚的含量。色谱柱:Thermo BDS HYPERSIL C18(4.6 mm×250 mm,5μm):流动相:甲醇-0.1%磷酸水溶液(80∶20);柱温:35℃;流速:0.8 mL/m in;检测波长:254 nm。结果:大黄酚在0.204~2.04μg范围内呈良好的线性关系,r=0.9999,平均回收率为98.15%,RSD=1.7%(n=6)。结论:该方法操作简便,专属性强,重现性好,结果准确可靠,可作为舒经跌打丸的质量控制方法。
Objective: To establish the quality standard of Shu Jing Dan Dao Pill. Methods: The qualitative identification of Sanqi medicinal materials in Shujing Guading Pills was carried out by thin layer chromatography. The content of chrysophanol in Shu Jing Guading Pills was determined by HPLC. Column: Thermo BDS HYPERSIL C18 (4.6 mm×250 mm, 5 μm): mobile phase: methanol-0.1% phosphoric acid aqueous solution (80:20); column temperature: 35°C; flow rate: 0.8 mL/m in; detection wavelength: 254 Nm. Results: Chrysophanol showed a good linear relationship in the range of 0.204~2.04μg, r=0.9999, the average recovery was 98.15%, and RSD was 1.7% (n=6). Conclusion: This method is simple, highly specific, reproducible, and accurate and reliable. It can be used as a quality control method for Shu Jing Dan Dao Pill.