论文部分内容阅读
目的了解血吸虫病血清学检测方法的联合应用的效果,为制定防治对策提供科学依据。方法选择46例慢性血吸虫病患者及42例健康人作为研究对象,采用间接血凝法(IHA)、金标渗滤法(DIGFA)、酶联免疫吸附试验(ELISA)分别检测其抗原和抗体,了解3种方法的敏感性与特异性,以及3种方法不同组合的检出阳性差别。结果IHA抗体检测敏感性为76.09%,特异性为95.24%;DIGFA抗体检测敏感性为69.57%,特异性为97.62%;ELISA循环抗原检测敏感性为32.61%,特异性为92.86%;任意2种方法阳性联合判定总的敏感性为65.22%,特异性为97.62%;3种方法同时阳性联合判定的敏感性为23.91%,特异性为100%。结论血吸虫病血清学检测循环抗原敏感性和特异性较差;任意2种方法联合阳性判定总的敏感性与特异性与单独IHA、DIGFA相似,无实际意义;检测抗体的IHA、DIGFA和检测循环抗原的ELISA3种方法联合阳性判定的敏感性与循环抗原ELISA相似,但特异性高,似与病原学诊断有相仿的意义,可在流行病学调查研究中结合运用。
Objective To understand the effect of combined application of serological test methods for schistosomiasis and provide a scientific basis for the development of control strategies. Methods Forty-six chronic schistosomiasis patients and 42 healthy volunteers were enrolled in this study. Antigen and antibody were detected by indirect hemagglutination (IHA), gold-labeled percolation (DIGFA) and enzyme linked immunosorbent assay (ELISA) To understand the sensitivity and specificity of the three methods, as well as positive detection of different combinations of the three methods. Results The detection sensitivity of IHA antibody was 76.09% and the specificity was 95.24%. The detection sensitivity and specificity of DIGFA antibody were 69.57% and 97.62%, 32.61% and 92.86% The sensitivity of the method was 65.22% and the specificity was 97.62%. The sensitivities of simultaneous determination of the three methods were 23.91% and 100% respectively. Conclusion The serological detection of schistosomiasis is less sensitive and specific for the detection of circulating antigen. The combined sensitivity and specificity of any two methods combined with positive IHA and DIGFA alone are of no practical significance. Detection of IHA, DIGFA and detection cycle The sensitivity of antigenic ELISA 3 methods in combination with positive determination is similar to that of circulating antigen ELISA, but the specificity is high, which may have the same meaning as etiological diagnosis, which can be combined with epidemiological investigation.