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作者报道了水仙叶大褐斑病的分布、危害性、症状和病原菌的研究结果。此病在上海崇明地区危害相当严重,感染指数30～80。病叶可提前4～8周枯死。初次侵染多发生于水仙叶尖端,褐色枯焦,再次侵染在叶和花梗上,叶片边缘及中部均可发生。病斑圆形、纺锤形、半圆形,褐色,周围发黄,严重时整叶枯死。病原菌与 Creager D.B.报道相符,为 Stagonospora curtisii(Berk)Sacc.分生孢子器球形或扁球形。分生孢子无色,圆筒形,单细胞或多细胞,变异甚大。以0.1%升汞水消毒0.5分钟,70%酒精消毒1分钟或无菌水洗8～9次表面消毒,均可分离培养成功。病原菌在20%水仙煎汁+2%蔗糖+2%琼脂培养基生长最好,而 PDA 培养基上比 PSA 培养基生长要好。此菌生长适温20～25℃,孢子萌发适温25℃,但13℃仍可萌发。多细胞孢子萌发好。病菌在 pH 4～8时均生长良好。以孢子悬浮液、菌丝块及病叶块进行接种试验,证明了此菌的致病性。
The authors report the distribution, harmfulness, symptoms and pathogens of narcissus grandiflora. The disease in Shanghai Chongming area is very serious harm, infection index 30 ~ 80. Sick leaves can die 4 to 8 weeks in advance. The first infection occurred in the tip of narcissus leaves, brown Kujiao, re-infection in the leaves and peduncle, leaf edge and the middle can occur. Round spot disease, fusiform, semicircular, brown, yellow around, serious when the entire leaf dead. Pathogenic bacteria and Creager D.B. reported consistent, Stagonospora curtisii (Berk) Sacc. Conidia spherical or oblate. Conidia colorless, cylindrical, single or multiple cells, great variation. Disinfection with 0.1% mercury chloride 0.5 minutes, 70% alcohol disinfection 1 minute or sterile water 8 to 9 times the surface disinfection can be isolated and cultured successfully. Pathogen grew best in 20% Narcissus Decoction + 2% sucrose + 2% agar medium, while PDA medium grew better than PSA medium. The growth of the bacteria suitable temperature 20 ~ 25 ℃, spore germination suitable temperature 25 ℃, but still 13 ℃ germination. Multicellular spores germinate well. The bacteria grew well at pH 4-8. Inoculation of spore suspension, mycelium and diseased leaves proved the pathogenicity of this bacterium.