论文部分内容阅读
目的探讨免疫炎症反应在大鼠弥漫性轴索损伤(DAI)中的作用及氢气对其保护作用。方法将96只SD雄性大鼠随机平均分为正常组、假手术组、DAI模型组(6h、1d、3d)和氢气干预组(6h、1d、3d)。采用头颅瞬间旋转损伤装置制作大鼠DAI模型,干预组每日2次腹腔注射高纯度氢气(10mL/kg)。各组于预定时间点通过HE染色、嗜银染色观察组织形态学的变化,GFAP免疫荧光染色观察胶质细胞增生活化情况,Western blotting分析炎症因子IL-6、IL-1β和信号通路JNK、p-JNK蛋白的表达。结果与假手术组比较,DAI顶叶皮髓交界区出现轴索肿胀、迂曲、轴索球等DAI特征性改变,GFAP阳性细胞数量增多(P<0.05),IL-6、IL-1β和p-JNK表达增高(P<0.01),氢气干预组较模型组脑组织形态学有所改善,GFAP阳性细胞数减少(P<0.05),IL-6、IL-1β和p-JNK表达降低(P<0.01)。结论免疫炎症反应参与了DAI急性期脑损伤的病理生理过程,星形胶质细胞的活化和炎症因子的大量释放导致脑损伤的加重。氢气能够通过减轻免疫炎症反应改善DAI急性期脑损伤。减轻免疫炎症反应程度对DAI急性期脑保护具有一定意义。
Objective To investigate the role of immunoinflammatory response in diffuse axonal injury (DAI) in rats and the protective effect of hydrogen. Methods Ninety-six male Sprague-Dawley rats were randomly divided into normal group, sham operation group, DAI model group (6h, 1d, 3d) and hydrogen intervention group (6h, 1d, 3d). Rat model of DAI was established by means of instantaneous head injury device. In the intervention group, high purity hydrogen (10 mL / kg) was injected intraperitoneally twice daily. The changes of histomorphology were observed by HE staining and argyrophilic staining at various time points. GFAP immunofluorescence staining was used to observe the proliferation and activation of glial cells. The inflammatory factors IL-6, IL-1β and JNK were detected by Western blotting, p-JNK protein expression. Results Compared with the sham-operation group, DAI showed morphological changes such as axonal swelling, tortuous, axial ball and the like, the number of GFAP positive cells increased (P <0.05), IL-6, IL- The expression of IL-6, IL-1β and p-JNK in hippocampus of rats in model group were significantly lower than those in model group (P <0.01) <0.01). Conclusion Immunoinflammatory reaction is involved in the pathophysiology of acute brain injury in DAI. The activation of astrocytes and the massive release of inflammatory cytokines lead to increased brain injury. Hydrogen can improve DAI acute brain injury by alleviating the immune inflammatory reaction. Mitigating the degree of immune inflammatory reaction has some significance on acute cerebral protection in DAI.