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为观察营养缺乏及补充维生素Ａ（ＶＡ）对胸腺细胞凋亡有关基因的影响，采用原位杂交及免疫组化的方法观察了胸腺组织ｂｃｌ－２ｍＲＮＡ，Ｆａｓ及Ｂａｘ蛋白的表达。结果表明营养缺乏的大鼠胸腺细胞ｂｃｌ－２ｍＲＮＡ的表达明显降低，而Ｆａｓ及Ｂａｘ蛋白的表达则明显增强；补充ＶＡ后大鼠胸腺ｂｃｌ－２ｍＲＮＡ的表达明显增强，Ｂａｘ蛋白的表达明显降低，而Ｆａｓ蛋白的表达无明显变化。本结果表明：营养缺乏时胸腺细胞凋亡增加导致免疫功能降低，补充ＶＡ可抑制细胞凋亡，可能是通过促进ｂｃｌ－２ｍＲＮＡ的表达，抑制Ｂａｘ蛋白的表达而起作用
To observe the effect of nutritional deficiencies and vitamin A (VA) on thymocyte apoptosis-related genes, the expression of bcl-2mRNA, Fas and Bax in thymus tissues was observed by in situ hybridization and immunohistochemistry. The results showed that the expression of bcl-2mRNA and the protein of Fas and Bax were significantly increased in the rat thymus, while the expression of bcl-2mRNA and the protein of Bax in thymus were significantly decreased Fas protein expression did not change significantly. The results show that: lack of nutrition thymus cells increased apoptosis led to reduced immune function, VA can inhibit apoptosis, may be through the promotion of bcl-2mRNA expression, inhibition of Bax protein expression and play a role