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用PCR法和DNA杂交法检测同一献血员的白细胞及血清中的HCMV DNA ,并用ELISA法检测血清中的HCMV IgM、IgG(测四个滴度 ) ,连续两年共检测白细胞和血清样本各 2 0 0人份。PCR法检测白细胞中的HCMV DNA阳性率分别为 6 3%和 70 % ,DNA杂交法检测的阳性率为42 %和 5 0 %。PCR法检测血清中的HCMV DNA的阳性率为 49%和 5 3% ,DNA杂交法检测的阳性率为 33%和 39%。HCMV IgM阳性率两年均为 5 %。HCMV IgG阳性率分别为 5 4%和 5 8%
Detection of HCMV DNA in leukocytes and serum from the same donator by PCR and DNA hybridization, and detection of HCMV IgM, IgG (four titers) in serum by ELISA, 2 leukocytes and 2 serum samples each for 2 consecutive years 0 0 people. The positive rates of HCMV DNA in leukocytes detected by PCR were 63% and 70% respectively, and the positive rates of DNA hybridization were 42% and 50%. The positive rates of HCMV DNA in serum by PCR were 49% and 53%, respectively. The positive rates of DNA hybridization were 33% and 39% respectively. HCMV IgM positive rate of 5% for both years. The positive rates of HCMV IgG were 54% and 58%