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弓形虫病是一种最为常见且遍布全球的传染病。虽然有多种免疫方法用于感染早期的诊断,但其有效性、敏感性、重现性和操作尚不令人满意。本文介绍了一种简便、经济的玻片微量免疫酶法(Micro-SIA)。 用鼠腹水中弓形虫RH株的速殖子,反复冻融制成1.5mg/ml蛋白的抗原,取抗原5ml包被24孔玻板,洗涤晾干,加待测血清
Toxoplasmosis is one of the most common and widespread infectious diseases in the world. Although a variety of immunological methods are used for early diagnosis of infection, their effectiveness, sensitivity, reproducibility and operation are not yet satisfactory. This article describes a simple and economical slide micro-immune enzyme method (Micro-SIA). With ascites Toxoplasma gondii RH strain of tachyzoites, repeated freezing and thawing made of 1.5mg / ml protein antigen, take antigen 5ml coated 24-well plate, washed and dried, plus test serum