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目的探讨可溶性晚期糖基化终末产物受体(sRAGE)水平及晚期糖基化终末产物受体(RAGE)基因单核苷酸位点多态性与2型糖尿病的关联。方法选择184例2型糖尿病患者作为糖尿病组,190例健康体检者作为正常对照组。ELISA方法检测sRAGE水平,PCR-Taq Man-MGB探针检测rs2070600、rs1800624、rs1800625及rs184003基因分型。结果糖尿病组sRAGE水平[M(P_(25),P_(75))]为1 087.4(713.5,1 213.4)pg/m L,正常对照组sRAGE为908.2(674.6,1 107.1)pg/m L,两组差异有统计学意义(P<0.001)。糖尿病组与正常对照组间4个位点的最小等位基因频率分布均无统计学差异,且在基因型分析中也无阳性发现。线性相关分析表明,4个位点与sRAGE水平均无明显关联性。结论 sRAGE水平与2型糖尿病的发病相关,但RAGE位点多态性与糖尿病无明显关联。RAGE位点多态性与sRAGE水平无关联。
Objective To investigate the association of soluble late glycation end product receptor (sRAGE) and single nucleotide polymorphisms of advanced glycation end-products receptor (RAGE) gene with type 2 diabetes mellitus (T2DM). Methods 184 cases of type 2 diabetes mellitus were selected as diabetic group and 190 cases of healthy subjects as normal control group. The sRAGE level was detected by ELISA and the genotypes of rs2070600, rs1800624, rs1800625 and rs184003 were detected by PCR-Taq Man-MGB probe. Results The level of sRAGE in diabetic group was 1 087.4 (713.5,1 213.4) pg / m L, while the sRAGE in control group was 908.2 (674.6,1 107.1) pg / m L [M (P 25, P 75) The difference between the two groups was statistically significant (P <0.001). No significant difference was found in the distribution of the allele frequencies among the four loci between the diabetic group and the normal control group, and no positive findings were found in the genotypes. Linear correlation analysis showed that there was no significant correlation between 4 sites and sRAGE levels. Conclusion The level of sRAGE is associated with the pathogenesis of type 2 diabetes. However, there is no significant association between polymorphism of RAGE and diabetes. There was no correlation between RAGE locus polymorphism and sRAGE level.