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目的比较粪便标本中艰难梭菌三种培养方法的差异性,寻找适合临床实验室使用的艰难梭菌培养方法。方法将健康儿童的300份粪便标本分别用快速芽孢孵育法、乙醇直接处理法、富集芽孢培养法进行培养,并对三种培养方法的差异性进行统计学比较。结果 300份粪便标本快速芽孢孵育法培养出艰难梭菌51例,检出率为17.0%;乙醇直接处理法培养出48例,检出率为16.0%;富集芽孢培养法培养出44例,检出率为14.7%。三种方法的检出率差异无统计学意义(P=0.7352,P>0.05)。结论乙醇直接处理法、快速芽孢孵育法简单快速,适合临床实验室进行艰难梭菌的快速培养;富集芽孢培养法杂菌少,适合大量艰难梭菌培养便于结果的观察及进一步的处理。
Objective To compare the differences of three culturing methods of C. difficile in stool specimens and to find a suitable C. difficile culture method suitable for clinical laboratory use. Methods 300 stool samples from healthy children were cultured by rapid spore incubation, ethanol direct treatment and enrichment spore culture respectively, and the differences of three culture methods were statistically compared. Results In the 300 spore specimens, rapid culture of C. difficile was carried out in 51 cases of C. difficile, the detection rate was 17.0%. 48 cases were cultured by ethanol direct method, the detection rate was 16.0%. 44 cases were cultured by enrichment spore culture, The detection rate was 14.7%. There was no significant difference in the detection rates of the three methods (P = 0.7352, P> 0.05). Conclusion Direct alcohol treatment and rapid spore incubation are simple and rapid, which are suitable for the rapid culture of Clostridium difficile in clinical laboratories. There are few bacteria in enrichment spore culture, suitable for a large number of Clostridium difficile culture to facilitate the observation and further treatment.