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目的:建立大鼠经皮肾穿刺造瘘模型,探讨血清胱抑素C在急性机械性肾损伤中的作用。方法:健康雄性Wistar大鼠共34只,其中10只行数字减影肾动脉造影(DSA),其余24只随机分为甲、乙、丙三组,每组8只,甲组(对照组)不穿刺不灌注,乙组(穿刺灌注组)以穿刺孔径1.6mm、灌注压力80cmH2O(1cmH2O=0.098kPa)行肾脏穿刺灌注;丙组(单肾组)术前行一侧肾脏切除后以1.6mm穿刺孔径、80cmH2O灌注压力行手术。于术后2h、12h、24h分别采血测定血清Cys C、BUN、SCr变化情况,术后取肾组织行病理检查。结果:大鼠行数字减影肾动脉造影,肾脏血管走形分布及集合系统形态结构显示清晰;同对照组相比,丙组术后2hCys C与术前相比有统计学意义(P<0.05);术后12h、24h血清Cys C、BUN、SCr与术前相比变化均有统计学意义(术后12hP<0.05;术后24hP<0.01)。结论:以大鼠建立经皮肾穿刺造瘘模型,可反映肾脏损伤情况;评价急性肾机械性损伤时,与SCr、BUN相比,CysC灵敏性更高。
Objective: To establish a rat model of percutaneous nephrostomy to investigate the role of serum cystatin C in acute mechanical injury. Methods: A total of 34 healthy male Wistar rats were randomly divided into digital subtraction renal artery angiography (DSA), and the other 24 were randomly divided into three groups: A, B and C, In group B (puncture and perfusion group), puncture of kidney was perforated with a puncture hole diameter of 1.6mm and a perfusion pressure of 80cmH2O (1cmH2O = 0.098kPa); group C (single kidney group) Puncture diameter, 80cmH2O perfusion pressure surgery. Serum levels of Cys C, BUN and SCr were determined by blood sampling at 2h, 12h and 24h after operation, respectively. Renal pathological examination was performed after operation. Results: Compared with the control group, the Cys C in group C at 2h after operation was significantly lower than that before operation (P <0.05) ). The changes of serum Cys C, BUN and SCr at 12h and 24h after operation were statistically different from those before operation (P <0.05 at 12h and 24h after operation). Conclusion: The model of percutaneous nephrostomy is established in rats, which can reflect the renal damage. When evaluating the mechanical injury of acute kidney, the sensitivity of CysC is higher than that of SCr and BUN.