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目的 探讨还原型谷胱甘肽对急性百草枯中毒的治疗作用。方法 在大鼠用百草枯 (Paraquat ,PQ)灌胃( 2 5 0mg/kg)染毒后不同时间腹腔注射还原型谷胱甘肽 (GSH) ,分别测定染毒后 8h、2 4h、48h、72h大鼠血浆和支气管──肺泡灌洗液 (BALF)中丙二醛 (MDA)含量及超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)的活力 ,并观察肺组织结构改变。结果 PQ染毒后血浆及BALF中MDA显著高于对照组 (P <0 .0 1) :血浆SOD活力下降 ,GSH Px48h后稍升高 ;BALF中SOD、GSH Px均下降 ,但前者 48h后升高 (P <0 .0 1)。给予GSH治疗后与中毒组相比 ,血浆和BALF中的MDA下降 (P <0 .0 5 ) ;SOD、GSH Px逐渐升高 ,但血浆SOD 72h似有下降。 4h、12h、2 4h三个时间点投用GSH的治疗组之间 ,SOD、MDA差异不大 ,仅 12h治疗组血浆中GSH Px稍低于 4h治疗组 (P <0 .0 5 ) ,BALF中GSH Px稍高于 4h治疗组 (P <0 .0 5 )。肺组织病理学检查可见PQ中毒后肺泡壁充血、增厚、炎症细胞浸润 ,并有局灶性出血 ;给予GSH后 ,肺泡壁充血、出血均有减轻。结论 给予还原型谷胱甘肽 ,可使急性百草枯中毒引起的脂质过氧化损害得到改善
Objective To investigate the therapeutic effect of reduced glutathione on acute paraquat poisoning. Methods Glutathione (GSH) was intraperitoneally injected into the stomach of rats with paraquat (PQ) intragastrically (250 mg / kg) for 8h, 24h, 48h, (MDA) content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) in the plasma and bronchial-bronchoalveolar lavage fluid (BALF) Lung tissue structure changes. Results The MDA level of plasma and BALF in PQ group was significantly higher than that in control group (P <0.01). The activity of SOD decreased in plasma and slightly increased after Px48h of GSH, while the levels of SOD and GSH Px decreased in BALF. However, High (P <0. 01). Compared with the poisoning group, the MDA in plasma and BALF decreased (P <0.05), the SOD and GSH Px gradually increased, but the plasma SOD decreased 72h. The levels of GSH Px in plasma of 12h and 12h treatment groups were slightly lower than those of 4h treatment group (P <0.05), while BALF GSH Px slightly higher than the 4h treatment group (P <0. 05). Pulmonary histopathology showed that alveolar wall hyperemia, thickening, infiltration of inflammatory cells and focal hemorrhage were found after PQ poisoning. After administration of GSH, alveolar wall hyperemia and hemorrhage were relieved. CONCLUSIONS: Reduced glutathione may improve lipid peroxidation induced by acute paraquat poisoning