目的：研究分析２所医院院内感染绿脓杆菌耐药小质粒同源性。方法：４６株耐药绿脓杆菌样本来自两个医院，其中甲院３４株，乙院１２株。发现甲院有１０株（２９．４％）携带质粒，乙院有４株带有质粒（３３．３％）。运用质粒转导技术和限制性内切酶谱分析。结果：８株（甲院６株，乙院２株）被证实为小耐药质粒，其中又有５株质粒（３．４ｋｂ或３．６ｋｂ）可诱导氯霉素耐药性，２株（２．５ｋｂ）可诱导卡那霉素耐药性，另有１株（４．５ｋｂ）可诱导对四环素耐药性。结论：推测２所医院部分耐药绿脓杆菌的小耐药质粒可能具有同源性，且又有所变异 OBJECTIVE: To study the homology of small-plasmid-resistant Pseudomonas aeruginosa infection in two hospitals. Methods: 46 drug-resistant Pseudomonas aeruginosa samples from two hospitals, including 34 cases of hospital, 12 cases of hospital. It was found that there were 10 (29.4%) carrying plasmids in the hospital and 4 in the hospital containing plasmids (33.3%). Using plasmid transduction technology and restriction endonuclease profiling. Results: 8 strains (6 in hospital and 2 in hospital) were confirmed as small resistant plasmids, of which 5 (3.4kb or 3.6kb) could induce chloramphenicol resistance and 2 2.5kb) can induce kanamycin resistance, and another one (4.5kb) can induce resistance to tetracycline. CONCLUSIONS: It is speculated that the small drug-resistant plasmids of some drug-resistant Pseudomonas aeruginosa in the two hospitals may have homology and have some variation