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目的:建立超高效液相色谱(UPLC)波长切换法同时测定桂龙咳喘宁片中芍药苷、盐酸小檗碱、药根碱、肉桂酸、桂皮醛5种成分的含量.方法:采用ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈(A)-0.1%甲酸溶液(B)为流动相,梯度洗脱(0~10 min,85%A;10~13 min,10%A;13~15 min,85%A);波长:0~1.8 min,230 nm;1.8~6.0 min,345 nm;6.0~9.0 min,285 nm;9.0~12.0 min,345 nm;流速:0.4 ml·min-1;柱温:30℃;进样量:5μl.结果:芍药苷、盐酸小檗碱、药根碱、肉桂酸、桂皮醛的线性范围分别是0.060~1.202μg(r=0.9999)、0.100~2.010μg(r=0.9999)、0.040~0.794μg(r=0.9994)、0.015~0.302μg(r=0.9999)、0.042~0.850μg(r=0.9999);平均加样回收率分别为99.63%、99.26%、100.17%、98.80%、100.26%,RSD分别为0.39%、0.97%、0.73%、1.00%、0.71%(n=6).结论:该方法操作简单,重复性好,可用于桂龙咳喘宁片的质量控制.“,”Objective:To develop a UPLC method for the simultaneous determination of 5 components in Guilong Kechuanning tablets including paeoniflorin, berberine hydrochloride, alkaloid, cinnamic acid and cinnamaldehyde. Methods:An ACQUITY UPLC BEH C18(2.1 mm ×100 mm,1.7 μm)chromatographic column was used;the mobile phase was acetonitrile(A)–0.1% formic acid solution (B) with gradient elution (0-10 min, 85% A;10-13 min, 10% A;13-15 min, 85% A) at a flow rate of 0. 4 ml·min-1, the detection wavelengths were:0-1. 8 min, 230 nm;1. 8-6. 0 min, 345 nm;6. 0-9. 0 min, 285 nm;9. 0-12. 0 min, 345 nm, and the column temperature was 30℃. Results:The linear range of paeoniflorin, berberine hydrochloride, alkaloid, cinnamic acid and cinna-maldehyde was 0. 060-1. 202 μg(r=0. 9999),0. 100-2. 010 μg(r=0. 9999),0. 040-0. 794 μg(r=0. 9994),0. 015-0. 302 μg(r=0.9999) and 0.042-0.850 μg(r =0.9999), the average recovery (n = 6) was 99.63%,99.26%,100.17%,98.80% and 100. 26%, and the RSDs were 0. 39%,0. 97%,0. 73%,1. 00% and 0. 71%, respectively. Conclusion:The method is simple, accu-rate and reproducible. It can be used for the quality control of Guilong Kechuanning tablets.