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The title structure of 14-O-[(4-amino-6-hydroxy-pyrimidine-2-yl)thioacetyl] mutilin, C_(26)H_(47)N_3O_5S, has been synthesized using 22-O-tosyl pleuromutilin and 4-amine-6-hydroxy-2-mercatopyrimidine monohydrate, and its structure was characterized by IR,