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目的:观察高原低氧条件下大鼠骨髓细胞凋亡情况,并应用沙利度胺进行干预研究,探讨细胞凋亡在慢性高原病发生发展中的作用。方法:TUNEL方法检测高原低氧条件下及其在沙利度胺干预后的大鼠骨髓细胞凋亡指数,与对照组进行比较分析。结果:高原低氧条件下1天时大鼠骨髓细胞凋亡指数减低,低氧1天组为(1.34±0.65)%,对照组为(2.73±0.51)%(P<0.01);与低氧21天组比较,沙利度胺干预后细胞凋亡增加,低氧21天组为(2.65±0.98)%,干预组为(4.02±2.13)%(P<0.05)。结论:高原低氧条件下大鼠骨髓细胞凋亡减少,细胞凋亡可能是慢性高原病的重要发病机制之一。抗血管新生药物沙利度胺有促进高原低氧大鼠骨髓细胞凋亡的作用,可能成为慢性高原病治疗的新手段。
OBJECTIVE: To observe the apoptosis of rat bone marrow cells under high altitude hypoxia and to investigate the effect of apoptosis on the occurrence and development of chronic mountain sickness by using thalidomide. Methods: TUNEL method was used to detect the apoptosis index of rat bone marrow cells under plateau hypoxia and thalidomide intervention, compared with the control group. Results: The apoptosis index of bone marrow cells of rats in one day after hypoxia was reduced (1.34 ± 0.65)% in hypoxia group and (2.73 ± 0.51)% in hypoxia group (P <0.01) Compared with the control group, the apoptosis increased after thalidomide intervention, the hypoxia 21 days group was (2.65 ± 0.98)%, and the intervention group was (4.02 ± 2.13)% (P <0.05). CONCLUSION: Apoptosis of rat bone marrow cells under hypoxia is reduced and apoptosis may be one of the important pathogenesis of chronic mountain sickness. The antiangiogenic drug thalidomide can promote the apoptosis of bone marrow cells in plateau hypoxia rats and may become a new treatment for chronic high altitude sickness.