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为研究不同UV-B辐射强度对烤烟生理代谢及调控途径的影响,应用蛋白质双向电泳联用质谱技术,以云南普遍种植的烤烟K326为试验材料,通过覆盖不同透明薄膜滤减UV-B辐射的方式,对75.8%(聚乙烯膜,处理1)和37.5%(麦拉膜,处理2)UV-B辐射透过率处理下K326的蛋白质组和相关生理性状进行了比较。结果表明:在蛋白质组中有10个蛋白在这两类处理下蛋白差异表达显著;与处理1相比,在处理2的K326叶片中有5个蛋白上调表达,5个蛋白下调表达;通过质谱分析共鉴定出8种功能明确的蛋白质,其中差异表达的10个蛋白中有3个与氧化还原相关,3个与光合作用相关,1个是参与能量代谢的激酶蛋白,1个是RNA结合蛋白,另外还有2个未知功能的蛋白待探明,在蛋白质组水平对不同UV-B辐射强度与烤烟生长发育的关系进行了初步研究;而在K326的生理成熟期、过渡期及工艺成熟期,处理2的净光合速率(Pn)均高于处理1,这与所鉴定出的3个与光合作用有光的蛋白在处理2中上调表达趋势一致;相比之下,处理1的K326发育进程较快,茎粗等形态指标及比叶重均比处理2高。
In order to study the effect of different UV-B radiation intensities on the physiological metabolism and regulation of flue-cured tobacco, protein-two-dimensional electrophoresis coupled with mass spectrometry was used to test the effect of UV-B radiation on the flue-cured tobacco. The proteome and related physiological traits of K326 were compared under 75.8% (polyethylene film, treatment 1) and 37.5% (Mylar, treatment 2) UV-B radiation transmissivity treatments. The results showed that there were 10 proteins in the proteome which were significantly different in these two treatments. Compared with treatment 1, 5 proteins were up-regulated and 5 proteins were down-regulated in K326 leaves of treatment 2, A total of eight proteins were identified, of which three were differentially expressed in redox, three were related to photosynthesis, one was kinase involved in energy metabolism and one was RNA-binding protein , And another two proteins with unknown function to be proved, the relationship between different UV-B radiation intensities and the growth and development of flue-cured tobacco at the proteome level was preliminarily studied; while in the physiological maturation, transitional and maturation stages of K326, , The net photosynthetic rate (Pn) of treatment 2 was higher than that of treatment 1, which was consistent with the identified three proteins with photosynthetic light up-regulated in treatment 2; in contrast, K326 development of treatment 1 The process is faster, stem diameter and other morphological indicators and specific leaf weight ratio of 2 high.