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目的:探讨腑安汤对术后肠麻痹的治疗作用及相关机制。方法:90只小鼠随机均分为假手术组、模型组和腑安汤预处理组后常规制作术后肠麻痹模型或行假手术。应用ELISA法测定小肠组织中类胰蛋白酶、组胺含量;光镜下观察经甲苯胺蓝染色的肠系膜肥大细胞计数及脱颗粒情况;利用比色法测定小肠组织中髓过氧化物酶(MPO)活性;检测小鼠肠道内碳末移行率。结果:与假手术组比较,模型组小肠组织类胰蛋白酶、组胺含量和MPO活性均明显升高(均P<0.01),而腑安汤预处理组小肠组织类胰蛋白酶、组胺含量和MPO活性的升高受到明显抑制,与模型组比较,差异均有统计学意义(均P<0.01);各组肥大细胞计数无统计学差异(P>0.05),但模型组细胞脱颗粒明显,而腑安汤预处理组较弱;术后模型组碳末移行率明显降低(P<0.01),腑安汤预处理组碳末移行率较模型组明显增加(P<0.01);相关性分析显示,MPO活性与碳末移行率呈负明显相关(r=-0.885,P<0.01)。结论:腑安汤可减少术后小鼠肠道类胰蛋白酶和组胺的产生,抑制肥大细胞脱颗粒及其所介导的炎症反应,有预防和减轻术后肠麻痹的作用。
Objective: To investigate the therapeutic effect of Fu-An Decoction on postoperative intestinal paralysis and related mechanisms. Methods: Ninety mice were randomly divided into sham-operation group, model group and Fu-An Decoction pretreatment group. The model of intestinal paralysis was performed routinely or sham operation was performed. The contents of tryptase and histamine in small intestine tissue were determined by ELISA. The mesentery mast cell count and degranulation were observed under light microscope. The levels of myeloperoxidase (MPO) Activity; detection of mouse intestinal carbon migration rate. Results: Compared with the sham operation group, the contents of trypsin, histamine and MPO in small intestine of model group were significantly increased (all P <0.01), but the contents of trypsin and histamine in small intestine of Fuan decoction (P <0.01). There was no significant difference in the number of mast cells between the two groups (P> 0.05). However, the degranulation of the model group was obvious, While the Fu-An Decoction preconditioning group was weaker. The carbon remnant migration rate was significantly lower in the model group after operation (P <0.01), and the carbon transfer rate in the Fuan Decoction pretreatment group was significantly higher than that in the model group (P <0.01). Correlation analysis The results showed that there was a negative correlation between MPO activity and carbon end-of-run rate (r = -0.885, P <0.01). Conclusion: Fu-An Decoction can reduce postoperative intestinal trypsin and histamine production, inhibit mast cell degranulation and inflammation mediated by it, and prevent and reduce postoperative intestinal paralysis.