【摘 要】
:
以墨兰的根状茎为外植体,研究培养基种类、NAA浓度和糖种类对墨兰根状茎增殖的影响.结果表明,当Hyponex1和Hyponex2单独使用时墨兰根状茎的生长及增殖效果不及MS培养基,但当H
【机 构】
:
延边大学长白山生物资源与功能分子教育部重点实验室,龙井市环保局,
论文部分内容阅读
以墨兰的根状茎为外植体,研究培养基种类、NAA浓度和糖种类对墨兰根状茎增殖的影响.结果表明,当Hyponex1和Hyponex2单独使用时墨兰根状茎的生长及增殖效果不及MS培养基,但当Hyponex1和Hy-ponex2混合使用时明显好于MS培养基.BA和NAA的混合使用有利于根状茎的增殖生长,当Hyponex-1-2培养基中BA 2 mg/L的条件下加入0.2 mg/L NAA时,根状茎分化数多且鲜物重和干物重良好.糖种类对根状茎增殖生长影响较大,在Hyponex-1-2+BA 2 mg/L+NAA 0.2 mg/L培养基中加入30 g/L蔗糖时增殖效果最佳,其次是葡萄糖,果糖不利于根状茎增殖培养.
Using Cymbidium rhizome as explant, the effects of medium type, NAA concentration and sugar type on the proliferation of Cymbidium chinense were studied.The results showed that when Hyponex1 and Hyponex2 were used alone, However, when Hyponex1 was mixed with Hy-ponex2, the proliferation of rhizomes was better than that of MS medium, and the combination of BA and NAA was beneficial to the proliferation and proliferation of rhizomes. mg / L NAA, the number of rhizomes differentiated more and the fresh weight and the dry weight were better.The sugar species had a great effect on the proliferation of rhizomes, but under the conditions of Hyponex-1-2 + BA 2 The best proliferation effect was obtained when 30 g / L sucrose was supplemented with 0.2 mg / L NAA and mg / L + NAA, followed by glucose, which was unfavorable for proliferation of rhizomes.
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