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在盆栽条件下,用外源脱落酸(Abscisic acid,ABA)处理块根形成期的木薯品种,测定处理及对照在不同时刻叶片中ABA等激素变化、ABA合成与失活以及淀粉合成途径关键基因的表达变化。结果表明:1)正常条件下木薯叶片内源ABA水平及ABA合成途径基因均存在明显的夜间低而白天较高的昼夜节律,ABA信号转导相关基因也表现出下午的高峰表达,它们与淀粉合成途径基因的夜间表达一般具有相反的趋势。2)ABA处理后,木薯叶片内源ABA含量迅速下降,与观察到的ABA合成途径基因表达下降与后期ABA失活基因激活表达相一致,同时发现细胞分裂素中的玉米素核苷(Zeatin riboside,ZR)和生长素(Indole acetic acid,IAA)水平发生改变,预示木薯具有迅速恢复ABA平衡态的机制,并且与其他类激素存在协同作用。3)与此同时,外源ABA处理明显抑制淀粉合成上游基因表达,特别是夜晚高峰期的表达,而对下游基因的抑制相对较小。说明这些基因及相关酶类不同程度受到ABA的调控;ABA明显倾向于调控淀粉合成上游相关基因;外源ABA处理改变了叶片临时淀粉合成速率。
Under potted condition, abscisic acid (ABA) was used to treat the cassava varieties in the root formation stage, and the changes of ABA and other hormones in the leaves at different time and ABA synthesis and inactivation, as well as the key genes of starch synthesis pathway Change of expression The results showed that: 1) The ABA level and ABA synthesis pathway genes of Cassava under normal conditions all had obvious low night-time and high day-night rhythm, and ABA signal transduction related genes also showed the peak expression in the afternoon, The nocturnal expression of synthetic pathway genes generally has the opposite trend. 2) After ABA treatment, the content of endogenous ABA in cassava leaves decreased rapidly, which was consistent with the observed decrease of ABA gene expression and later ABA inactivation gene expression. Meanwhile, the expression of Zeatin riboside , ZR) and IAA (IAA), indicating that cassava has the mechanism of rapid recovery of ABA equilibrium and synergies with other hormones. 3) At the same time, exogenous ABA treatment significantly inhibited the upstream starch synthesis gene expression, especially at night peak, while the downstream gene inhibition was relatively small. Indicating that these genes and related enzymes are regulated by ABA to varying degrees; ABA obviously tends to regulate the upstream starch synthesis genes; and exogenous ABA treatment changes the rate of temporary starch synthesis.