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目的采用高效液相色谱法测定蒺藜不同药用部位中总黄酮醇苷的含量,并对蒺藜茎、叶、果实、全草中总黄酮醇苷含量进行比较。方法以槲皮素、异鼠李素、山奈酚为对照,采用高效液相法,色谱条件:ODS色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.4%磷酸溶液(50:50),检测波长:360 nm。结果蒺藜不同药用部位中总黄酮醇苷的含量有较大差异,其中茎、叶、果实、全草中总黄酮醇苷含量分别为2.19 mg/g、8.15 mg/g、1.67 mg/g、4.36 mg/g,叶中含量最高,果实中含量最低;槲皮素、异鼠李素、山奈酚在线性范围(分别为0.0312~0.312μg、0.0204~0.204μg、0.0112~0.112μg)内,呈良好的线性关系(依次r=0.9999、r=0.9998、r=0.9999);回收率试验的RSD分别为0.92%、1.61%、1.44%。结论本研究所建立的分析方法准确可行,可用于蒺藜不同药用部位中总黄酮醇苷含量测定。
OBJECTIVE To determine the content of total flavonol glycosides in different medicinal parts of Tribulus terrestris by high performance liquid chromatography (HPLC), and to compare the contents of total flavonol glycosides in stem, leaf, fruit and whole plant of Tribulus terrestris. Methods Quercetin, isorhamnetin and kaempferol were used as control. The chromatographic conditions were as follows: ODS column (250 mm × 4.6 mm, 5 μm), mobile phase: methanol-0.4% phosphoric acid solution 50), detection wavelength: 360 nm. Results The contents of total flavonol glycosides in different medicinal parts of Tribulus terrestris varied greatly. The contents of total flavonol glycosides in stem, leaf, fruit and whole plant were 2.19 mg / g, 8.15 mg / g and 1.67 mg / g, 4.36 mg / g, the highest content in leaf, the lowest in fruit; Quercetin, isorhamnetin and kaempferol were in the linear range of 0.0312 ~ 0.312μg, 0.0204 ~ 0.204μg, 0.0112 ~ 0.112μg (R = 0.9999, r = 0.9998, r = 0.9999, r = 0.9999, respectively). The RSD of recovery test were 0.92%, 1.61% and 1.44% respectively. Conclusion The analytical method established in this study is accurate and feasible and can be used for determination of total flavonol glycosides in different medicinal parts of Tribulus terrestris.