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目的探讨补中益气汤对免疫激发态小鼠免疫功能的调节作用。方法将经卵清蛋白免疫过的小鼠40只分A、B两组,分别灌服补中益气汤和葡萄糖,1次/d,每次0.5 ml/只,连续7 d,第8天将所有小鼠再次加强免疫,再经7 d,采血分离血清,用ELISA法检测血清中相应抗体的生成水平。结果补中益气汤组小鼠抗体生成水平明显低于葡萄糖组,差异有统计学意义(P<0.01)。结论补中益气汤可以下调小鼠的免疫应答水平,降低抗体生成。
Objective To investigate the regulatory effect of Buzhong Yiqi Decoction on immune function in immune-challenged mice. Methods A total of 40 mice immunized with ovalbumin were divided into two groups: A group and a B group. The mice were given 0.5ml per decoction of Bu Zhong Yi Qi Decoction once a day for 7 days on the 8th day All mice were boosted again, and after 7 days, the blood was collected to secrete serum, and the level of the corresponding antibody in serum was detected by ELISA. Results The level of antibody production in Buzhong Yiqi decoction group was significantly lower than that in glucose group (P <0.01). Conclusion Buzhongyiqi Decoction can reduce the level of immune response in mice and reduce antibody production.