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Carcia等(1983)报道检测隐孢子虫卵囊最有效的浓缩方法是Sheather’s溶液漂浮法,且若用相差显微镜观察制备的标本无需染色。随后相继发展了一些净化隐孢子虫卵囊的技术。迄今,应用最广泛的较新方法有Percoll不连续密度梯度分离法和使用由Zierdt(1984)发明的寄生虫浓缩装置。但由于在卵囊悬液中含有大量的污染物,使用这些方法不能获得良好的效果。作者在寻找分离卵囊的新方法时发现,用ConcanavalinA-Sepharose 4B代价昂贵,而用Percoll连续密度梯度离心分离则较快速,并且能回收原样本中80％的卵囊。至今在试验过的所有染色方法中,对粪便样本中诊断最有效的方
Carcia et al. (1983) reported that the most effective concentration method for detecting Cryptosporidium oocysts was Sheather’s solution floatation method, and no staining was required if prepared by phase contrast microscopy. Followed by the development of some of the technology to purify Cryptosporidium oocysts. To date, Percoll discontinuous density gradient separation and the use of the parasite concentration device invented by Zierdt (1984) have been the most widely used newer methods. However, due to the large amount of contaminants contained in the oocyst suspension, good results can not be achieved with these methods. When looking for a new method of isolating oocysts, the authors found that Concanavalin A-Sepharose 4B was expensive, whereas Centrifugation was faster with Percoll serial density gradient centrifugation and recovered 80% of the original oocysts. So far in all the staining methods tested, the most effective way to diagnose in stool samples