从野生豆科植物根瘤中分离根瘤菌,是研究、开发和利用我国生物固氮资源的一个重要方面。1982年在辽宁省铁岭地区15个县采集了143份野生大豆根瘤材料,剔除霉变的12份,其余131份材料接种于铁丰18号大豆种子做重新结瘤试验。从中进行了分离,得到31个菌株,对其中19株做了进一步纯化和初步生理鉴定以及水培结瘤试验。对此19株分离物还做了耐酸度比较,鉴定结果见表1。在此基 Isolation of rhizobia from the wild legume nodules is an important aspect of research, development and utilization of biological nitrogen fixation resources in our country. In 1982, 143 wild soybean nodule materials were collected from 15 counties in Tieling, Liaoning Province, 12 pieces of mildew were removed, and the remaining 131 pieces of materials were inoculated on the iron seeds of Tiefeng 18 to regrow the nodules. 31 strains were isolated, of which 19 strains were further purified and preliminary physiological identification and hydroponic nodulation test. 19 isolates also made acid-resistant comparison, the identification results in Table 1. In this base