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运用随机扩增多态性DNA（RandomamplifiedpolymorphicDNA，RAPD）技术对发生于中国东北的大豆发斑病菌（Cercosporidiumsojinum）的10个生理小种进行基因组DNA多态性分析。用13个10-核苷酸随机引物共计获得了111个RAPD标记，其中86.5％具有多态性，通过聚类分析确定了供试小种间的亲缘关系。试验证明，RAPD技术分析大豆灰斑病菌遗传变异可提供大量分子标记，综合分析13个随机引物的扩增谱带可将供试菌株清楚分开。RAPD技术是一项操作简单、快速和灵敏的方法，极具对病菌群体遗传分析的潜力。
Ten races of Sercosporidium sojinum from northeastern China were genotyped by genomic DNA using random amplified polymorphic DNA (RAPD) technique. A total of 111 RAPD markers were obtained using 13 10-nucleotide random primers, of which 86.5% were polymorphic. The genetic relationship among tested races was determined by cluster analysis. Experiments show that RAPD analysis of C. Solani genetic variation can provide a large number of molecular markers, a comprehensive analysis of 13 random primers amplification bands can be clearly divided test strains. RAPD technology is a simple, fast and sensitive method that has great potential for genetic analysis of germ-line populations.