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目的探讨农药敌匹硫磷和残杀威以及环境内分泌干扰物双酚A对小鼠RAW264.7细胞增殖活性的联合毒作用类型。方法采用噻唑蓝(MTT)比色法检测细胞活性。通过加权机率单位法计算敌匹硫磷、残杀威与双酚A以及它们的混合物(受试物按等毒性比混合)各自的半数生长抑制浓度(IC50)及其95%CI。采用Marking相加指数法、等效应线图解法和Logistic回归分析法判断敌匹硫磷与双酚A、残杀威与双酚A的联合细胞毒作用类型。结果染毒24h后,敌匹硫磷、残杀威与双酚A对RAW264.7细胞的IC50及其95%CI分别为194.1μg/ml(173.4～217.4μg/ml)、448.4mg/L(358.2～573.2μg/ml)和37.5μg/ml(35.3～39.9μg/ml),而敌匹硫磷与双酚A混合物、残杀威与双酚A混合物的IC50及其95%CI分别为168.8μg/ml(160.1～178.2μg/ml)、253.4μg/ml(236.0～273.0μg/ml)。联合毒性评价结果显示,3种评价方法均将敌匹硫磷与双酚A联合作用类型判断为拮抗作用,而将残杀威与双酚A联合作用类型判断为相加作用。结论敌匹硫磷与双酚A对RAW264.7细胞增殖活性的联合毒作用类型为拮抗作用,而残杀威与双酚A联合毒作用类型为相加作用。
Objective To investigate the combined toxicity of pesticide parathion and propoxur and environmental endocrine disruptors Bisphenol A on the proliferation of mouse RAW264.7 cells. Methods Cell viability was measured by MTT assay. The respective half-maximal growth inhibitory concentrations (IC50) and their 95% CIs of antiseptophan, propoxur and bisphenol A and their mixtures (test compounds mixed in equal toxicity ratios) were calculated by the weighted probability unit method. Marked additive index method, the equivalent line diagram method and Logistic regression analysis to determine the enemy of parathion and bisphenol A, propoxur and bisphenol A combination of cytotoxic effects. Results IC50 values and their 95% CIs of VP, BP and BPA in RAW 264.7 cells were 194.1 μg / ml (173.4-217.4 μg / ml) and 448.4 mg / L (358.2 mg / ~ 573.2μg / ml) and 37.5μg / ml (35.3 ~ 39.9μg / ml), respectively. The IC50 and its 95% CI of the mixture of propoxur and BPA were 168.8μg / ml (160.1 ~ 178.2μg / ml), 253.4μg / ml (236.0 ~ 273.0μg / ml). The results of the joint toxicity evaluation showed that all three evaluation methods judged antagonistic action of the combination of parathion and bisphenol A, and judged the combination of propoxur and bisphenol A as the additive effect. Conclusion The combination of antagonistic effect of proplatoxid and bisphenol A on the proliferation of RAW264.7 cells is antagonistic, while the combination of propoxur and bisphenol A is additive.