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目的 研究淫羊藿总黄酮对雄性大鼠生殖内分泌功能的影响。方法 (1)观察淫羊藿总黄酮对未成年大鼠生殖器官重量的影响。淫羊藿总黄酮经灌胃给药7天后,腺垂体、睾丸、附睾及精囊腺称重;(2)观察相同剂量的淫羊藿总黄酮对不同年龄大鼠睾酮、雌二醇和黄体生成素的影响,睾酮、雌二醇和黄体生成素水平用放免法测定;(3)观察淫羊藿总黄酮在大鼠睾丸间质细胞体外培养中对睾酮的影响。结果 淫羊藿总黄酮可增加未成年大鼠腺垂体、附睾及精囊腺重量,提高睾酮、雌二醇和黄体生成素水平,能明显促进离体大鼠间质细胞睾酮基础分泌。结论 研究表明,淫羊藿总黄酮对雄性生殖系统和生殖内分泌的功能具有促进作用。
Objective To study the effects of total flavonoids of Epimedium on the reproductive and endocrine functions of male rats. Methods (1) To observe the effect of total flavonoids of Herba Epimedii on the weight of reproductive organs in juvenile rats. After 7 days of total flavonoids of Epimedium, the pituitary gland, testis, epididymis and seminal vesicles were weighed; (2) Observed the same dose of total flavonoids of Epimedium on testosterone, estradiol and luteinizing hormone in rats of different ages. The effects of testosterone, estradiol, and luteinizing hormone levels were measured by radioimmunoassay. (3) Observed the effects of total flavonoids of Epimedium on testosterone in vitro of rat Leydig cells. Results Epimedium total flavonoids can increase the weight of adenohypophysis, epididymis and seminal vesicles in immature rats, increase the levels of testosterone, estradiol and luteinizing hormone, and significantly promote the secretion of testosterone in isolated rat stromal cells. Conclusions Studies have shown that total flavonoids of Epimedium can promote the functions of the male reproductive system and reproductive endocrine.