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1985年夏季在我院核桃树上发现双齿绿刺蛾[Latoia hilarat(Staudinger)]自然罹病死亡幼虫。经扫描电镜观察,发现有大量圆形颗粒,结合透镜观察确认是一株颗粒俸病毒(GV)。经回接试验证明,再分离与原接种病原的形态特征一致,并具有较高的毒力。幼虫的感病症状是后期虫体肿胀,小幼虫几乎近球泡形,虫体表皮一触即破。将分离提纯的病原在扫描电镜下观察,颗粒体长约400—500毫微
In the summer of 1985 found in our hospital walnut tree bidentata green larvae [Latoia hilarat (Staudinger)] natural death larvae. Scanning electron microscopy and found that a large number of round particles, combined with lens observation confirmed that it is a particle pan-pen virus (GV). Back-up test proved that the re-isolation consistent with the morphological characteristics of the original vaccination and has high virulence. Symptoms of larvae is late swollen body swollen, small larvae almost spherical shape, a touch of the worm’s epidermis broken. Separation and purification of the pathogen observed under a scanning electron microscope, particle length of about 400-500 nanometers