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目的建立茯蒲洗液中蛇床子素含量测定的高效液相色谱法。方法采用反相高效液相色谱法,以日本DL-C18柱(4.6mm×150mm,5μm)为色谱柱,甲醇-水(65∶35)为流动相,检测波长322nm,流速为1.0mL/min。结果蛇床子素在浓度为4~100μg/mL范围内有良好的线性关系,相关系数为0.9994,精密度相对标准偏差(RSD)为1.27%,平均加样回收率为98.32%,RSD为1.17%。结论本方法操作简单、快速、准确,可用于茯蒲洗液中蛇床子素的含量测定。
OBJECTIVE To establish HPLC method for the determination of osthol in Fupao lotion. Methods The mobile phase consisted of DL-C18 column (4.6 mm × 150 mm, 5 μm) and methanol-water (65:35) as mobile phase at a detection wavelength of 322 nm at a flow rate of 1.0 mL / min using reversed-phase high performance liquid chromatography . Results The osthole had a good linear relationship in the concentration range of 4 ~ 100 μg / mL, the correlation coefficient was 0.9994, the relative standard deviation (RSD) was 1.27%, the average recovery was 98.32% and the RSD was 1.17% . Conclusion The method is simple, rapid and accurate and can be used for the determination of Osthole in Fu-Po lotion.