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目的:制备硅纳米球包裹的Ru(bpy)_3~(2+)(SiO_2@Ru)作为电致化学发光(electrogenerated chemiluminescence,ECL)探针的生物传感器,评价其在丙型肝炎核心抗原(HCVcAg)检测中的应用价值。方法:采用“油包水”的微乳剂方案,制备SiO_2@Ru纳米复合物,纳米粒子粒径一致,因此具有很好的重复性,应用戊二醛作为交联剂实现抗体与硅球的交联。SiO_2@Ru可作为ECL探针,通过夹心法检测HCVcAg。结果:HCVcAg检测的线性范围为1~1 000 fmol/L,最低检出限为3 fmol/L。与临床HCV RNA比较,有较好的相关性,线性相关系数R~2=0.937 9。结论 :构建HCVcAg的检测的硅纳米免疫传感器具有很高的灵敏度和很好的重复性,可用于临床诊断、疗效评价以及血液中心的血源筛查。
OBJECTIVE: To prepare the biosensor of Ru (bpy) _3 ~ (2 +) (SiO_2 @ Ru) coated with silicon nanospheres as a probe of electrogenerated chemiluminescence (ECL) ) Detection of the value of the application. Methods: The microemulsion solution of “water in oil” was used to prepare SiO_2 @ Ru nanocomposite with the same particle size. Therefore, glutaraldehyde Of the cross-linked. SiO_2 @ Ru can be used as an ECL probe to detect HCVcAg by sandwich method. Results: The linear range of HCVcAg assay was 1 ~ 1 000 fmol / L and the limit of detection was 3 fmol / L. Compared with the clinical HCV RNA, there is a good correlation, the linear correlation coefficient R ~ 2 = 0.9379. Conclusion: The detection of HCVcAg silicon nano-immunosensor with high sensitivity and good reproducibility, can be used for clinical diagnosis, evaluation of efficacy and blood center blood screening.