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目的 了解谷氨酸钠对铝吸收的影响。方法 1 8只 SD雄性大鼠 ,体重 (2 50±1 5) g,被翻转的离体十二指肠、空肠、回肠 ,在其内腔充满 p H 7.3的缓冲液 (其组成为 Tris- HCl1 5.5mmol,Na Cl1 2 0 .7mmol,KCl5.6mmol,Ca Cl2 2 .5mmol,Mg Cl2 1 .2 mmol,glucose1 1 .5mmol)后 ,置于 p H7.3含待测液的生理盐水中 ,待测物分别为 2 0 mmol Al Cl3,2 0 mmol Al Cl3+2 0mmol柠蒙酸钠和 2 0 mmol Al Cl3+2 0 mmol谷氨酸钠。恒温 (37± 0 .1 )℃培养 0 .5h,培养期间通入95% O2 和 5% CO2 气体。然后用石墨炉无火焰原子吸收分光光度计 ,分别测定肠内腔液和肠的铝含量。结果 对照组 (Al Cl3)十二指肠、空肠、回肠对铝的吸收率 (鲜肠重计 )分别为 3.59± 0 .55,3.41± 0 .61和 3.1 9± 0 .68(μg/g) ,参照组 (Al+柠檬酸钠 )分别为 4.92± 0 .73,4.47± 0 .56和4.37± 0 .77(μg/g) ,试验组 (Al+谷氨酸钠 )分别为 4.75± 0 .68,4.45± 0 .59和 4.32± 0 .63(μg/g) ,参照组与试验组的十二指肠、空肠、回肠铝吸收率与对照组相比有显著差异。如将肠段重换算成干重 ,则参照组和试验组仅十二指肠铝的吸收率与对照组相比有显著差异 (其吸收率为 :2 7.51±1 .88和 2 8.67± 2 .1 6对 2 4 .98± 1 .75(μg/g) ,而空肠、回?
Objective To understand the effect of sodium glutamate on aluminum absorption. Method 1 Eight SD male rats, weighing (250 ± 15) g body weight, were dissected from the isolated duodenum, jejunum and ileum, and filled in their lumen with a buffer of pH 7.3 (consisting of Tris- HCl1 5.5 mmol, NaCl1 2 0 .7 mmol, KCl5.6 mmol, CaCl2 2 .5 mmol, MgCl2 1 .2 mmol, glucose1 1 .5 mmol), placed in p H7.3 physiological saline containing the test solution, The analytes were 20 mmol AlCl3, 20 mmol AlCl3 + 20 mmol sodium citrate and 20 mmol AlCl3 + 20 mmol sodium glutamate respectively. The culture was incubated at 37 ± 0 .1 ° C for 0.5 h, and 95% O2 and 5% CO2 gas were introduced during the incubation. Then graphite furnace flame atomic absorption spectrophotometer, respectively, determination of intestinal lumen and intestinal aluminum content. Results The absorption rates of aluminum (duodenum, jejunum and ileum) in the control group (Al Cl3) were 3.59 ± 0.55, 3.41 ± 0.61 and 3.1 9 ± 0.68 (μg / g ), Reference group (Al + sodium citrate) were 4.92 ± 0.73, 4.47 ± 0.56 and 4.37 ± 0.77 (μg / g), respectively, and the test group (Al + glutamate) was 4.75 ± 0 respectively. 68,4.45 ± 0 .59 and 4.32 ± 0.63 (μg / g) respectively. There was a significant difference in absorption rate of aluminum in the duodenum, jejunum and ileum between the reference group and the experimental group compared with the control group. If the intestines were re-calculated as dry weight, the absorption rate of aluminum in the duodenum of the reference group and the experimental group was significantly different from that of the control group (absorption rates: 2 7.51 ± 1.88 and 2 8.67 ± 2 .1 6 vs 24.498 ± 1 .75 (μg / g), whereas the jejunum, back?