为了探索甜瓜细胞核雄性不育系ms5花药败育发生的时期及不同时期雄花物质含量与雄性不育之间的关系,采用甜瓜雄性不育两用系不育株ms5及可育株mf5雄花为研究材料,分别开展花药发育结构的电镜观察,并且对各时期雄花生理生化特性进行物质含量的测定。电镜观察显示ms5花粉育性为0,mf5花粉活力高达98%以上;不育株与可育株花药在四分体时期出现明显差异,可育株花粉囊开裂,花粉粒溢出,而不育株花粉囊干瘪,无开裂迹象。不育株各个时期丙二醛含量均高于可育株(开花前期除外),不育株可溶性糖含量都低于同时期的可育株,不育株蛋白质含量及游离脯氨酸含量均在花药发育四分体时期出现转折变化,说明不育株花器官存在物质能量代谢降低现象,物质元素的积累与甜瓜败育相关。 In order to explore the relationship between anther abortion of ms5 and the content of male flower in different stages of male sterility of male sterile line MS5 in melon, the male sterility of melon male sterility ms5 and male fertility mf5 were studied Materials, respectively, to carry out the electron microscopic observation of anther development structure, and the physiological and biochemical characteristics of male flowers at various stages of the determination of the substance content. The results of electron microscopy showed that the ms5 pollen fertility was 0 and the mf5 pollen viability was as high as 98%. The anthers of sterile plants and fertile plants showed significant differences in the tetrad stage. The fertile pollens broke down and the pollen grains spilled, while the sterile plants Wrinkles dry, no signs of cracking. The malondialdehyde content of the sterile plants was higher than that of the fertile plants (except pre-flowering), the soluble sugar content of the sterile plants was lower than that of the fertile plants at the same period, the protein content and free proline content of the sterile plants were in the Anther developmental tetrad period turning point changes, indicating that the sterile organs of flower matter energy metabolism phenomenon, the accumulation of material elements and melon abortion.