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目的观察基于化痰化瘀法的三贝地甲煎对多囊卵巢大鼠的雄激素含量的影响。方法连续注射30 g·L~(-1)脱氢表雄酮(DHEA,2μL·g~(-1))20 d,诱导大鼠建立多囊卵巢模型。将造模后的大鼠分为5组:空白组(灌服生理盐水)、对照组(灌服炔雌醇环丙孕酮,1.8μg·g~(-1))、二甲双胍组(每日0.225 g)、2个剂量实验组(每日灌服中药18,4.5 g,10μL·g~(-1))。15 d后,处死动物,比较各组体重,切片观测卵巢组织学指标,酶联免疫吸附法检测各组的雄激素值。结果与空白组相比,高剂量实验组能够控制多囊卵巢大鼠的体重增长(P<0.01),明显改善其卵巢形态学变化(P<0.01),明显降低其血清雄激素值(P<0.01),综合作用优于炔雌醇环丙孕酮或二甲双胍。结论三贝地甲煎中药复方能控制多囊卵巢模型大鼠的体重增长,调控其雄激素值,改善其卵巢形态。
Objective To observe the effect of “San Bei Di Jia Jian” on the androgen content of polycystic ovary rats based on the method of resolving phlegm and removing blood stasis. Methods The polycystic ovary model was established by continuous injection of 30 g · L -1 dehydroepiandrosterone (DHEA, 2 μL · g -1) for 20 days. The rats were divided into 5 groups: the blank group (irrigated with saline), the control group (fed with ethinyl estradiol cyproterone, 1.8 μg · g -1), the metformin group 0.225 g), two doses of experimental group (daily oral administration of Chinese medicine 18,4.5 g, 10μL · g -1). Fifteen days later, the animals were sacrificed and the body weight of each group was compared. The ovarian histological indexes were observed and the androgen values of each group were detected by enzyme-linked immunosorbent assay. Results Compared with the blank group, the high dose group could control the weight gain of the polycystic ovary rats (P <0.01), significantly improve the morphological changes of the ovary (P <0.01) and significantly lower the serum androgen levels (P < 0.01), the combined effect is better than ethinyl estradiol cyproterone or metformin. Conclusion Three Bei Di Jia Jian Chinese herbal compound can control the weight gain of polycystic ovary model rats, regulate its androgen value, and improve its ovarian morphology.