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目的:研究酸浆醇提物(ethanol extracts of Physalis alkekengi Linn.,EEP)对食管癌EC-1.71细胞增殖的抑制作用,探讨其可能的作用机制。方法:EEP作用于EC-1.71细胞后,采用倒置显微镜观察细胞形态的改变;采用细胞计数法观察其对细胞增殖的抑制作用;通过流式细胞仪检测法分析EEP对EC-1.71细胞周期的影响。结果:EEP作用后,EC-1.71细胞出现了形态变化,细胞生长受到抑制,且呈时间和浓度依赖性,细胞周期被阻滞在S期。结论:EEP对食管癌EC-1.71细胞具有增殖抑制作用。
OBJECTIVE: To study the inhibitory effect of ethanol extracts of Physalis alkekengi Linn. (EEP) on the proliferation of esophageal carcinoma EC-1.71 cells and to explore its possible mechanism. Methods: After EC was treated with EEP, the morphological changes of EC-1.71 cells were observed by inverted microscope. The inhibitory effect of EEP on cell proliferation was observed by cell counting. The effect of EEP on the cell cycle of EC-1.71 cells was analyzed by flow cytometry . Results: The morphological changes of EC-1.71 cells were observed after treated with EEP. The growth of EC-1.71 cells was inhibited in a time-and-concentration-dependent manner. The cell cycle was arrested in S phase. Conclusion: EEP can inhibit the proliferation of EC-1.71 cells.