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用纳米金溶胶与聚乙烯醇缩丁醛(PVB)构成复合固酶基质,采用溶胶凝胶法固定葡萄糖氧化酶(GOx)于铂金电极表面,并在葡萄糖溶液中加入天青Ⅰ作为电子媒介体,制成了新型葡萄糖生物传感器。实验证明,葡萄糖氧化酶吸附在纳米金颗粒表面上稳定且保持其生物活性;而电子媒介体的存在,显著提高了传感器的响应灵敏度。该传感器对葡萄糖响应的线性范围为2.5×10-5～7.5×10-3mol/L;检出限为8.5×10-6mol/L(S/N=3)。该生物传感器用于人体血清中的葡萄糖测定,结果令人满意。
Nano gold sol and polyvinyl butyral (PVB) constitute a composite enzyme-immobilized matrix. The glucose oxidase (GOx) was immobilized on the surface of platinum electrode by sol-gel method. Azure I was added to the glucose solution as an electron mediator , Made a new glucose biosensor. Experiments show that glucose oxidase adsorption on the surface of gold nanoparticles stable and maintain its biological activity; and the presence of electronic media significantly improve the sensitivity of the sensor response. The linear response of the sensor to glucose was in the range of 2.5 × 10-5 ~ 7.5 × 10-3mol / L with the detection limit of 8.5 × 10-6mol / L (S / N = 3). The biosensor was used for the determination of glucose in human serum with satisfactory results.