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采用甲苯胺蓝染色和免疫组织化学ABC方法,对大鼠舌组织中肥大细胞的分布、形态特点以及细胞内的P物质、血管活性肠肽和神经肽Y的定位进行了研究.结果表明,大鼠舌组织内肥大细胞主要分布于固有层和肌层,多呈圆形、椭圆形或长梭形.经与甲苯胺蓝染色的相邻切片比较,几乎所有肥大细胞分别呈P物质、血管活性肠肽和神经肽Y免疫反应阳性,提示,舌组织肥大细胞内P物质、血管活性肠肽和神经肽Y等生物活性肽可能与其局部血液供应及感觉密切相关.
Tolueneamine blue staining and immunohistochemical ABC method were used to study the distribution and morphology of mast cells in rat tongue tissue and the localization of intracellular P substance, vasoactive intestinal peptide and neuropeptide Y. The results showed that large Mast cells in murine tongue tissue mainly distributed in the lamina propria and muscular layer, mostly round, oval or long fusiform.Compared with adjacent sections stained with toluidine blue, almost all mast cells were substance P, vasoactive Intestinal peptide and neuropeptide Y immunoreactivity, suggesting that the tongue mast cells, substance P, vasoactive intestinal peptide and neuropeptide Y and other bioactive peptides may be closely related to its local blood supply and feeling.